Real-Time multiplex PCR test CE IVD-marked and designed to detect genomic DNA of Pneumocystis jirovecii.

Pneumocystis jirovecii is a common cause of pneumonia in individuals with compromised immune systems.

This organism cannot be cultured in vitro and consequently Real-Time PCR is indicated among conventional diagnostic procedures to combat Pneumocystis pneumonia.

PneumID® is validated on DNA extracted from fungal cultures and clinically relevant respiratory specimens:
– BAL (bronchoalveolar lavage)

The kit is compatible with the most common DNA extraction and Real-Time PCR instruments.

  • Sensitivity <1 jirovecii genome.
  • Detection in a range of 6 orders of magnitude (target no. <1 to 10^6)
  • Result in <1h after DNA extraction.
Test REAL-TIME PCR multiplex, marcato CE IVD e progettato per rilevare il DNA genomico di Pneumocystis jirovecii. da BAL e Broncolavaggio alveolare

How to best combat Pneumocystis jirovecii pneumonia

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PneumID is a multiplex qPCR assay designed to detect genomic DNA of Pneumocystis jirovecii from human respiratory samples. Pneumocystis jirovecii is a common cause of potentially fatal pneumonia in individuals with compromised immune systems. This organism cannot be cultured in vitro, and consequently laboratory detection relies primarily on microscopic identification directly from patient specimens using fluorescent markers or antibodies. The lack of sensitivity of this method led to the proposal of Pneumocystis-specific Real Time PCR (qPCR) as an additional tool to aid conventional diagnostic procedures for Pneumocystis pneumonia by providing sensitive and specific detection of Pneumocystis jirovecii DNA in clinical specimens.

The kit is validated to detect Pneumocystis jirovecii DNA present in direct samples: BAL (bronchoalveolar lavage) and broncholavage.

It starts with a minimum volume of 200ul of BAL from which DNA extraction is performed in a final volume of 50ul.

PneumID uses the most widely used qPCR chemistry, which is based on the detection of light emitted by hydrolysis probes, detected through different fluorescent channels. The kit contains individual primers and probes designed for the detection of genomic DNA of Pneumocystis jirovecii, human beta-globin and an exogenous internal control.

PneumID is a multiplex Real Time PCR kit capable of simultaneously detecting genomic DNA of Pneumocystis jirovecii, an exogenous extraction control and an endogenous control (human beta-globin). The result provided by the PneumID assay is qualitative.

The choice to use a qualitative assay and an endogenous control stems from the nature of the starting material used for this type of analysis: in fact, the gold standard is bronchoalveolar lavage (BAL), a diluted respiratory sample that cannot be standardized (in fact, the dilution coefficient cannot be traced with certainty). The use of an endogenous control allows the “cellularity” of the sample to be traced, providing an indication of the quality of the sample and making it possible to avoid false-negative results due to unsuitable sampling.

PneumID is an “open” kit in that it can be used on the most common instruments found in laboratories.
We mention only a few of them.

EXTRACTION: Qiagen EZ1, QiaSymphony, Qiagen QIAamp spin column, Promega Wizard Genomic Kit, Qiagen QiaCube, Roche MagnaPure and others.

AMPLIFICATION: ABI 7500, BMS MIC, Qiagen RotorGene, BioRadCFX96, Illumina Eco, Roche LC480 (II) and others.

PneumID enables a diagnosis in a short time, allowing the clinician to initiate specific therapy. The correct use of PneumID involves the evaluation and interpretation of the result in relation to the patient’s clinical context. Pneumocystis jirovecii is in fact a fungus that can give colonization of the airways, but without developing pathology: the evaluation of the patient’s clinical picture is therefore fundamental in the interpretation of the data obtained from the PneumID test.

The diagnosis of Pneumocystis jirovecii pneumonia when the Real Time PCR result is weakly positive can be supported by positive serum Beta-D-Glucan values.